. 2023 Jan 26;22:18. doi: 10.1186/s12934-022-02009-7

Table 1.

Comparison of the reaction medium composition at the end of the Fed batch flask and the bioreactor P. pastoris cultures expressing LevB₁SacB under the GAP promoter, and the reaction using LevB₁SacB produced by E. coli

	Time (h)	L-FOS/Sucrose (g/ g)	Glucose (g)	Fructose (g)	Residual sucrose (g)	Maximum L-FOS DP	Total P. pastoris biomass (g)
X-33/pGAPZαA-LevB₁SacB (Flask-level)	288	0.28	0	0	0	20	1.05
X-33/pGAPZαA-LevB₁SacB (Bioreactor scale)	59	0.45	0	0	6.83	10	21.85
Rosetta 2/PET-22 ( +) LevB₁SacB *	6	0.41	54 g/L	234 g/L	65 g/L	10	–

Time (h)

L-FOS/Sucrose (g/ g)

Glucose (g)

Fructose (g)

Residual sucrose (g)

Maximum L-FOS DP

Total P. pastoris biomass (g)

X-33/pGAPZαA-LevB₁SacB

(Flask-level)

288

0.28

1.05

X-33/pGAPZαA-LevB₁SacB

(Bioreactor scale)

0.45

6.83

21.85

Rosetta 2/PET-22 ( +) LevB₁SacB *

0.41

54 g/L

234 g/L

65 g/L

–

Data taken from Porras-Domínguez, 2017