FIG. 2.

(A) Identification of parasite-derived As14 in A. suum at various developmental stages. The parasite extract was prepared essentially as described in Materials and Methods. Twenty micrograms of parasite extract or ES products was separated by SDS-PAGE, and the proteins were then transferred to a nitrocellulose membrane. Lane 1, rAs14 (50 ng); lane 2, L3; lane 3, lung-stage larvae; lane 4, female worm; lane 5, male worm; lane 6, larval ES; lane 7, female worm ES; lane 8, male worm ES. The antigen bound to the mouse anti-rAs14 serum was detected using NBT-BCIP. (B) Expression of As14 homologues in ascarid nematodes. Lane 1, A. lumbricoides; lane 2, T. canis; lane 3, A. suum; lane 4, rAs14 (50 ng). Forty micrograms of protein equivalents of each parasite extract was used for immunoblot analysis with mouse anti-rAs14 serum.