FIG. 1.

Ig binding of ECOR strains. Whole-cell extracts from 24-h broth cultures were fractionated by SDS-PAGE (8% acrylamide) and blotted to polyvinylidene difluoride (PVDF). The blot was incubated sequentially with human IgG Fc-HRP (B) and IgA-HRP (A). Lanes: 1, ECOR-2; 2, ECOR-5; 3, ECOR-9; 4, ECOR-12; 5, ECOR-43; 6, ECOR-72. Each lane contained approximately 30 μg of protein.