M9 medium glucose

Reagent	Final concentration	Amount
Calcium chloride (CaCl2) solution	0.1 mM	0.1 mL
Magnesium sulfate (MgSO4) solution	2 mM	1 mL
Wolfe’s vitamin solution (1,000×)	1×	1 mL
Trace element solution (2,000×)	1×	0.5 mL
M9 medium salts (10×)	1×	100 mL
Glucose monohydrate	40 mM	7.2 g
Double deionized water	N/A	Up to 1 L
Total	N/A	1 L