Protocol for absolute quantification of proteins in Gram-negative bacteria based on QconCAT-based labeled peptides

. 2023 Jan 24;4(1):102060. doi: 10.1016/j.xpro.2023.102060

Trace element solution (2,000×)

Reagent	Final concentration	Amount
Disodium EDTA	15 g/L	15
Zinc sulfate (ZnSO₄) heptahydrate	4.5 g/L	4.5
Manganese chloride (MnSO₄) tetrahydrate	0.7 g/L	0.7
Cobalt(II) chloride (CoCl₂) hexadrate	0.3 g/L	0.3
Copper(II) chloride (CuCl₂) dihydrate	0.2 g/L	0.2
Disodium molybdate (Na₂MoO₄) dihydrate	0.4 g/L	0.4
Calcium chloride (CaCl₂) dihydrate	4.5 g/L	4.5
Iron(II) sulfate (FeSO₄) heptahydrate	3.0 g/L	3.0
Boric acid (H₃BO₃)	1.0 g/L	1.0
Potassium iodide (KI)	0.1 g/L	0.1
Double deionized water	N/A	Up to 1 L
Total	N/A	1 L