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. Author manuscript; available in PMC: 2023 Jan 27.
Published in final edited form as: J Vis Exp. 2022 May 18;(183):10.3791/64091. doi: 10.3791/64091

Figure 1. Comparison of lifespan measurements with and without sterilization.

Figure 1.

(A) Lifespans of wildtype N2 nematodes grown on NGM-agar plates seeded with empty vector (ev) or hsf-1 RNAi bacteria at 20 °C. Animals were moved away from progeny on day 1, 3, 5, and 7 of adulthood. (B) Lifespans of wildtype N2 nematodes grown on NGM-agar-FUDR plates seeded with empty vector (ev) or hsf-1 RNAi bacteria at 20 °C. Animals were grown to adulthood on standard ev or hsf-1 RNAi plates, then moved to FUDR plates at day 1 adulthood. (C) Lifespans of glp-4(bn2) mutant animals grown on NGM-agar plates seeded with empty vector (ev) or hsf-1 RNAi at 25 °C. For all conditions, animals were scored for death every 2 days until all animals were recorded as dead or censored. Animals with bagging, protrusion or explosion of vulva, or those that crawled up the sides of the plates and desiccated were censored. All statistics were performed using Log-Rank Mantel Cox testing and can be found in Table 2.