Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Visual record of the line: normal	Fig. 1 panel A
Phenotype	Qualitative analysis: Immunofluorescence staining	Positive expression of pluripotency markers: Oct3/4, Nanog, Sox2	Fig. 1 panel C
	Quantitative analysis: RT-qPCR	NANOG and SOX2 expressed in the iPSC lines and absent in differentiated CMs	Fig. 1 panel D
Genotype	Whole genome array (KaryoStat™ Assay) Resolution 1–2 Mb	Normal karyotype: SCVIi063-A 46, XX SCVIi064-A 46, XY	Supplementary Fig. 1 panel A
Identity	STR analysis	16 loci tested match well	Submitted in archive with authors
Microbiology and virology	Mycoplasma	Mycoplasma testing by luminescence: Negative	Supplementary Fig. 1 panel B
Differentiation potential	Directed differentiation	Positive IF staining of three germ layer markers	Fig. 1 panel E
Germ layer markers	Expression of these markers demonstrated at mRNA (RT-PCR) and protein (IF) levels	Ectoderm: Pax6, Otx2 Endoderm: Sox17, Foxa2 Mesoderm: Brachyury, Tbx6	Fig. 1 panel E
Mutation analysis	Sequencing	Heterozygous	Fig. 1 panel F