Fig.3. Effects of circ_0000345 overexpression on ox-LDL-induced ASMC proliferation, migration, invasion, and inflammatory response.

(A) RT-qPCR verifying the transfection efficiency of the circ_0000345 overexpression plasmid. (B–I) ASMCs with or without ox-LDL stimulation and circ_0000345/pCD-ciR-transfected ASMCs with ox-LDL stimulation were used. (B) MTT assays showing the viability of ASMCs. (C) Western blotting analysis showing the relative levels of PCNA protein in ASMCs. (D) Flow cytometry assays exhibiting the apoptosis of ASMCs. (E) Western blotting analysis showing the relative levels of Cleaved caspase-3 in ASMCs. (F and G) Wound healing and transwell invasion assays exhibiting the migration and invasion of ASMCs. (H and I) The release of proinflammatory cytokines TNF-α and IL-6 in ASMCs. ^＊P＜0.05.