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. 2022 Sep 29;29(12):1849–1863. doi: 10.5551/jat.63327

Supplementary Fig.1. Effects of circ_0000345 silencing on cell proliferation, migration, invasion, and inflammatory response in ox-LDL-stimulated ASMCs.

Supplementary Fig.1. Effects of circ_0000345 silencing on cell proliferation, migration, invasion, and inflammatory response in ox-LDL-stimulated ASMCs

(A-I) ASMCs were transfected with si-NC or si-circ_0000345 and then treated with ox-LDL. (A) Relative expression of circ_0000345 in the above treated ASMCs. (B) The viability of the above treated ASMCs was determined by MTT assay. (C) Relative protein levels of PCNA in the above treated ASMCs were detected by western blotting. (D) The apoptosis of the above treated ASMCs was detected via flow cytometry assay. (E) PCNA protein levels in the above treated ASMCs were measured via western blotting. (F and G) Cell migration and invasion in the above treated ASMCs were evaluated by wound healing and transwell invasion assays. (H and I) ELISA was used to measure the release of TNF-α and IL-6 in the above treated ASMCs. P<0.05.