Table 1. Primer sequences used for RT-qPCR.
Genes | Primer sequences (5’-3’) |
---|---|
circ_0000345 | Forward (F): 5’-ATGTGGCCTTCCAAACCATCC-3’ |
Reverse (R): 5’-AGAACATTGGCTGTAGAACGG-3’ | |
PAPD5 | F: 5’-CTCCATGCGGCCTCGTC-3’ |
R: 5’-TCGTCTCTAGGGGCAGGAAG-3’ | |
miR-647 | F: 5’-GCCGAGGTGGCTGCACTCACT-3’ |
R: 5’-AGCAGGGAGATAACGGACTGAC-3’ | |
GAPDH | F: 5’-GACAGTCAGCCGCATCTTCT-3’ |
R: 5’-GCGCCCAATACGACCAAATC-3’ | |
U6 | F: 5’-CTCGCTTCGGCAGCACA-3’ |
R: 5’-AACGCTTCACGAATTTGCGT-3’ | |
RSF1 | F: 5’-TACTCCACAGTTGATGAAGGCA-3’ |
R: 5’-CATCTCCCATGCCCAGGTACT-3’ |