TABLE 3.
Entry into murine macrophages
| Time (min) after entry | % Infected cellsa
|
No. of vacuoles/cellb
|
No. of bacteria/vacuolec
|
Infectivity indexd
|
||||
|---|---|---|---|---|---|---|---|---|
| M. marinum | M. smegmatis | M. marinum | M. smegmatis | M. marinum | M. smegmatis | M. marinum | M. smegmatis | |
| 60 | 17.4 ± 0.91 | 7.0 ± 0.21 | 1.96 ± 0.04 | 1.18 ± 0.02 | 6.23 ± 0.17 | 1.32 ± 0.05 | 19.49 ± 0.90 | 1.0 ± 0.90 |
| 120 | 24.3 ± 0.20 | 9.1 ± 0.60 | 1.97 ± 0.10 | 1.14 ± 0.20 | 9.85 ± 0.29 | 1.12 ± 0.12 | 40.58 ± 0.29 | 1.0 ± 0.60 |
a
Percentage of cells containing at least one bacterium in a field. Results are the means ± standard deviations for three counts of 25 fields.
b
Number of bacterial vacuoles in each cell. Results are the means ± standard deviations for two counts of 50 cells in different sections of the same preparation.
c
Number of bacteria in each vacuole. Results are the means ± standard deviations for two counts of 50 cells in different sections of the same preparation.
d
The infectivity index is calculated as follows: (% infected cells × number of vacuoles per cell × number of bacteria per vacuole for M. marinum)/(% infected cells × number of vacuoles per cell × number of bacteria per vacuole for M. smegmatis).