Figure 5. PA modulation of the anti-inflammatory pathways in the adipose tissue significantly delays PDAC tumor growth.
(A) Heat map showing hierarchical clustering of genes from the adipose tissue of control and KrasG12D mice after a DIO intervention of a HFD or HFD+PA from Figure 2A. Blue = low expression, red = high expression. (B) Gene ontology displaying top significant pathways modulated based on adipose tissue gene expression due to PA from Figure 2A cohort (C) Fold change adipose tissue gene expression of IL-15ra in control and KrasG12D mice from Figure 2A, analyzed via Mann-Whitney test. (D) 9-week old Female C57BL/6J mice (n=10/group, 5/cage) received an empty/control or IL-15 adipocyte-targeting rAAV vector and were implanted with tumors 3 weeks later. (E) Fold change adipose tissue IL-15 gene expression at the end of the study, analyzed via Mann-Whitney test. (F) Representative IVIS images and (G) quantified fold change average photon radiance signal of mice with and without IL-15 adipose targeting, analyzed via two-way ANOVA after log transformation.