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[Preprint]. 2023 Jan 12:2023.01.07.523111. [Version 2] doi: 10.1101/2023.01.07.523111

Figure 4: Lentiviral transduction induced silencing of ENKUR gene in CD34+ stem cells.

Figure 4:

(A) Flow cytometric analysis of cell surface markers to confirm the megakaryocytic and platelet fractions after 15 days of culture. (B) RNA expression levels in the CD34+ cell-derived megakaryocytes and platelets. (C) (i) Western blot of the CD34+ cells derived megakaryocytes (n=4 cord blood and n=4 peripheral blood) and (ii) densitometry for the blots. Densitometry was performed using the IVIS imaging software (****p<0.0001, ***p<0.001, **p<0.01 and *p<0.05 when compared with non-silenced controls).