Table 1.

Sequence parameters for IDP constructs

Parameter	Aap-PGR	SasG-PGR	Aap-Arpts	SdrC-SD	SD-30mer
N	135	69	189	62	30
f-	0.15556	0.13043	0.22222	0.33871	0.50000
f+	0.1037	0.23188	0.06878	0.08065	0
FCR	0.25926	0.36232	0.29101	0.41935	0.50000
NCPR	−0.05185	0.10145	−0.15344	−0.25806	−0.50000
Kappa	0.05825	0.09562	0.08655	0.30207	0.02324
SCD	0.79	1.11	15.80	4.06	10.79
FPR	0.28889	0.17391	0.09524	0.03226	0
Omega	0.03234	0.07106	0.05146	0.00657	0.00096
Hydropathy	3.09259	2.72899	3.08466	2.64839	2.35
Phase Plot Region	2	3	2	3	4

The CIDER server [24] was used to calculate most parameters, including those required for the Das-Pappu Plot [4]; SCD was calculated as described [23].

N: Number of residues

f-: Fraction of negative residues

f+: Fraction of positive residues

FCR: Fraction of charged residues

NCPR: Net charge per residue

Kappa: κ is a charge patterning parameter [4]. Highly mixed charged sequences approach κ = 0, while highly segregated charged sequences approach κ = 1.

SCD: Sequence charge decoration [23]. Well-mixed charged sequences approach SCD = 0, whereas highly segregated charged sequences show large values of SCD.

FPR: Fraction of proline residues (not a parameter provided by CIDER, but included here for relevance)

Omega: Ω is a charge/proline patterning parameter [25]. This parameter is similar to κ, but also incorporates proline residues. If prolines and charged residues are well mixed along a sequence (with respect to other amino acids), there will be a low Ω value. If proline/charged residues are highly segregated, Ω will approach 1.

Hydropathy: Based on the Kyte-Doolittle scale [26], normalized from 0 (least hydrophobic) to 9 (most hydrophobic).

Phase Plot Region: Location on the Das-Pappu phase plot this sequence falls

Phase Plot Annotation:

1: Weak polyampholytes and polyelectrolytes (Globules & Tadpoles)

2: Boundary region (Janus sequences)

3: Strong polyampholytes

4: Strong negatively charged polyelectrolytes 5: Strong positively charged polyelectrolytes