Table 3.
Sedimentation velocity AUC parameters
| IDP | Mseq (kDa) |
s
20,w
(dc/dt) |
Mexp (kDa) (dc/dt) |
s20,w (c(s)) |
f/f0 (c(s)) |
Mexp (kDa) (c(s)) |
Rs (Å) |
Rh (Å) (SEC) |
Rh (PPII charge) |
|---|---|---|---|---|---|---|---|---|---|
| Aap-PGR | 13.1 | 1.02 | 14.3 | 1.06 | 2.12 | 14.4 | 33.9 | 37.1 | 37.84 |
| SasG-PGR | 7.5 | 0.76 | 8.9 | 0.80 | 2.03 | 9.3 | 28.1 | 24.8 | 24.43 |
| Aap-Arpts | 19.1 | 1.25 | 25.6 | 1.30 | 2.52 | 22.2 | 46.2 | 40.8 | 44.06 |
| SdrC-SD | 6.4 | 0.91 | 8.4 | 0.97 | 1.93 | 8.1 | 24.7 | 21.1 | 22.15 |
Data were analyzed using the -dc/dt fitting in DCDT+ and by SEDFIT’s continuous c(s) distribution model [39] and are presented in Figure 3. The Stokes-Einstein radius, Rs, is from SEDFIT, Rh was determined experimentally by SEC, and the predicted hydrodynamic radius, Rh (PPII charge), is from Table 2, shown here for comparison.