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[Preprint]. 2023 Jan 9:2023.01.09.522931. [Version 1] doi: 10.1101/2023.01.09.522931

Figure 1. PF4 and KKO inhibits fibrin generation initiated by NET and DNA fragments.

Figure 1.

Fibrin generation in pooled normal plasma (PNP) with or without added HMW, digested NETs (blue), or DNA (purple). DN1 = DNase I, Af2 = AflII, BsGrI-HF = BG1 and Al1 = AluI. Data are mean ± SEM) of at least 3 independent experiments as indicated in each bar. (A) Lag time determined from kinetic curves of fibrin generation with NETs (blue) and DNA (purple). (B) Slope (rate) of fibrin generation was determined from the same kinetic curves as in (A). (C) Lag time of DNA-induced fibrin generation was determined from kinetic curves similarly to (A), in the presence of PF4 (20 μg/mL). (D-E) Lag times are shown for fibrin generation studies of DNase I-digested NETs (D) and DNA (E) in the absence or presence of 1 μg/mL low PF4 and/or 10 μg/mL KKO. Data are mean ± SEM of at least 3 independent experiments as indicated in each bar.