FIG. 2.

Typical experiment showing the effect of T-cell depletion on the cytokine-stimulating activities of the mycobacterial chaperonins as assessed by assay of IL-6 and IL-8 synthesis. PBMC were depleted of CD3 cells by rosetting with the RosetteSep reagent from StemCell Technologies. The depletion was assessed by flow cytometry (a), and the effect of depletion on IL-6 and IL-8 production by the remaining cell population was measured (b). Results are expressed as the means ± standard errors of triplicate cultures. ▧, nondepleted cells; ▪, depleted cells. PolyB, polymyxin B.