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[Preprint]. 2023 Feb 28:2023.01.14.524039. Originally published 2023 Jan 14. [Version 3] doi: 10.1101/2023.01.14.524039

Figure 1.

Figure 1.

CandyCollect efficiently captures S. mutans and S. aureus and facilitates quantitative bacterial detection by qPCR. (Ai) Fluorescence microscopy images of S. mutans and S. aureus before elution (left), and after elution with Proteinase K in ESwab™ buffer (ESB) (middle) and with Proteinase K (Pro. K) in PBS (right). (Aii) Quantification of fluorescence microscopy images. Each data point represents data from one CandyCollect device and is the average of the integrated density per area of 12 (10000 μm2) regions of interest (ROI) from 4 images of the CandyCollect device (3 ROIs per image). The bar graph represents the mean ± SEM of n = 3 CandyCollect devices. Data sets were analyzed using one-way ANOVA with Tukey’s multiple comparison test; p-values are indicated for pairwise comparisons: ***p=0.0009, ****p<0.0001. (B) Reported concentration of S. mutans (left) and S. aureus (right) from qPCR analysis of bacteria eluted from CandyCollect devices using proteinase K in PBS as the CandyCollect elution buffer. Bacteria were incubated in-lab at concentrations of 103, 104 and 105 CFU/mL. S. mutans and S. aureus were fluorescently labeled with WGA.