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[Preprint]. 2023 Feb 28:2023.01.14.524039. Originally published 2023 Jan 14. [Version 3] doi: 10.1101/2023.01.14.524039

Figure 4.

Figure 4.

Shelf life tests demonstrate that CandyCollect devices effectively capture S. pyogenes after 1 year of storage. Devices were plasma treated and stored at room temperature for 0 days (control group), 3 months, 4 months, and 1 year. (A) Fluorescence microscopy images indicate capture of S. pyogenes after 1 year of storage is similar to the control, with ~25% decrease in bacteria captured. (B) Quantification of the integrated density per area (pixel/μm2). Data sets were analyzed using one-way ANOVA and Tukey’s multiple comparison tests (*p<0.05). No significant difference between 0 days (control group) and 3 months of storage was found. Note: depicted images are from CandyCollect devices incubated with S. pyogenes at a concentration of 1 ×109 CFU/mL for 10 minutes. Each data point represents an individual CandyCollect device (4 images were taken per device, and the data point plotted is the average); the bars represent the mean ± SEM of n=3 CandyCollects. S. pyogenes was fluorescently labelled with WGA.