Statistics characterizing the GeneMarkS-T initial gene predictions made in assembled transcripts as well as the HC genes predicted by the GeneMarkS-TP module. Two versions of a reference protein database were used for each species: the ‘Species excluded’, as well as the ‘Order excluded’ (see Data Sets). Refinement made by GeneMarkS-TP reduced the number of initial predictions and produced a significant increase in Pr. In most of the genomes there was also a positive change in Sn, especially in large inhomogeneous genomes of G. gallus and M. musculus. Additional data is provided in Supplemental Table S1.