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[Preprint]. 2023 Jan 13:2023.01.12.523820. [Version 1] doi: 10.1101/2023.01.12.523820

Fig. 1. Multiomic single-nucleus analyses identify diverse cortical cell types in the dorsolateral prefrontal cortex of controls and C9orf72 ALS/FTD patients with different levels of pTDP-43.

Fig. 1.

(A) Schematic representation of single-nucleus multiome profiling (snATAC-seq and snRNA-seq in the same nuclei) of dorsolateral prefrontal cortex samples from 5 control and 9 c9orf72 ALS/FTD donors analyzed in this study. (B) pTDP-43 levels in control and C9orf72 ALS/FTD patient cortical tissues. (C) snATAC-seq and snRNA-seq integrated UMAP visualization of major cortical cell types where each dot corresponds to each of the 52,334 nuclei profiled simultaneously for transcriptome and chromatin accessibility using the 10x multiome platform. (D) Row-normalized single-nucleus gene expression (left) or gene score (right) heatmaps of cell-type marker genes. (E) Pseudo-bulk chromatin accessibility profiles for each cell type at cell-type marker genes.