Figure 6. Depletion of WASp, N-WASp or DIAPH1 neither affects RPA or β-actin protein expression nor ssDNA formation.

A. WB and quantitation showing protein levels of RPA2 and β–actin upon depletion of N-WASp, DIAPH1 or WASp. Bar charts represent mean +/− SEM of three experiments and statistical significance was determined using t test.

B. Representative images and dot plots of BrdU signal intensity per nucleus of Hela cells treated with control siRNA (siCTRL) or siRNA targeting N-WASp, DIAPH1 or WASp upon HU treatment at the indicated dose/duration (red lines indicate mean values). Cells were incubated with BrdU (10 μM) for 1h prior to HU treatment. Dot plots represent data pooled from two independent experiments. Statistical significance was determined using the Mann-Whitney test. n.s., nonsignificant.