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[Preprint]. 2023 Jan 20:2023.01.19.524731. [Version 1] doi: 10.1101/2023.01.19.524731

Figure 1. Anti-α4β7 treatment is associated with decreased frequency of naïve B and T cells in the intestines of patients with UC.

Figure 1.

a, Representative flow cytometry (FC) plots for total (non-PC) B cells (CD45+CD19+CD38−), switched memory B cells (CD45+CD19+CD38−IgM−IgD−) and naïve B cells (CD45+CD19+CD38−IgM+IgD+) from the ileum of a UC patient pre- and post-VDZ. b, Frequency of total B cells, naïve B cells and switched memory B cells from ileum and colon of untreated (n=34), VDZ-treated (n=21) or TNFi-treated UC patients (n=10) (top panels). Longitudinal analysis of paired biopsy samples from UC patients taken pre- and post-VDZ therapy (bottom panels). c, Representative FC plots showing total plasma cells (CD27+CD38++) and their expression of CD45 and CD19 in the left colon of a UC patient pre- and post-VDZ. d, Frequency of total plasma cells, short-lived (CD45+CD19+) and long-lived (CD45+CD19−) plasma cells in terminal ileum and left colon of UC patients. e, Representative FC plots showing the expression of CD4 and CD8 on CD3+ T cells (left panel) and the expression of CD45RA on CD4 T cells (right panel) in the ileum of a UC patient pre- and post-VDZ. f, Frequency of CD3 T cells, CD4 and CD8 T cells (top panel), as well as naïve (CD45RA+) CD4 and CD8 T cells and CD4 to CD8 ratio (bottom panel). Top panels: data are shown as individual data and mean. Non-parametric analysis was done using Kruskal-Wallis test and Dunn’s multiple comparison test, p values are indicated. Bottom panels: paired non-parametric analysis was done using Wilcoxon test, p values are indicated.