Figure 5. IDR structural preferences divide between weak and strong entropic force.

(A) Entropic force vs. the number of residues in 94 different IDRs. The black curve is an exponential fit of GS-repeat data. Each point represents the entropic force of a single sequence calculated from 5 independent repeats. The color-coding shows the entropic difference between the IDR and the same-length GS-repeat $\left(\overline{\mathrm{\Delta }S}=\mathrm{\Delta }S/\mathrm{\Delta }{S}^{GS}-1\right)$, with purple (green) markers showing a stronger (weaker) entropic force compared to the equivalent GS-repeat. (B) Entropic force vs the GS-repeat normalized end-to-end distance ${\overline{R}}_{ee}$ (see Eq. 3). Each marker represents a single IDR color-coded as in (A). (C) XZ-projections of C_α density for 3 different IDRs with increasing asphericity. The constraint plane is normal to Z=0 such that the density at Z>0 will avoid the surface and the density at Z<0 clashes with the surface (the disallowed region is indicated by the red color).