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[Preprint]. 2023 Jan 31:2023.01.22.525042. Originally published 2023 Jan 22. [Version 2] doi: 10.1101/2023.01.22.525042

Figure 4. Establishing the Local Cysteine Oxidation (Cys-LOx) method to analyze basal mitochondrial cysteine oxidation states.

Figure 4.

A) Scheme of Cys-LOx method. B) Percent oxidation state of mitochondrial cysteines identified with mito-Cys-LOx. C) Percent oxidation state of cysteines quantified in an exemplary mitochondrial protein. D) Difference in redox states of cysteines quantified with Mito-Cys-LOx with or without dialyzed FBS and CHX treatment. Red dots indicate cysteines localized in mitochondria. Black dots indicate cysteins localized in organelles other than mitochondria. Dialyzed FBS (Dia-FBS) treatment was 36 h and CHX treatment was 100 ug/mL for 6 h at 37 °C. Experiments were performed in triplicate in iBMDM cells. All MS data can be found in Table S4.