Figure 4. Each cortical layer of the neocortex displays a unique transcriptional impact of sleep deprivation.

A. Prediction score of the deconvolution step for each of the 2085 spots of a representative example slice for each cortical layer are represented with the color legend from blue to red: layer 2–3 (A₁), layer 4 (A₂), layer 5 (A₃), layer 6 (A₄). We can distinguish between distinct sequential laminar excitatory neurons layers on the aggregated profile (A₅). B. UpSet plot of interactions between each deconvoluted cortical layers of the neocortex. The number of DEGs submitted for each layer is represented by the histogram on the left (0–225 range). A gene is significant if its FDR step-up < 0.001 and its log2fold-change ≥ |0.2|. Dots alone indicate no overlap with any other lists. Dots with connecting lines indicate one or more overlap of DEGs between cortical layers. The number of DEGs in a specific list of overlap is represented by the histogram on the top. Genes are labeled for the smallest lists. L2/3 = Layer 2 and 3 ; L4 = Layer 4 ; L5 = Layer 5 ; L6 = Layer 6. The unique lists of 174 DEGs for layer 5 and 149 DEGs for layer 2/3 that enrich specific molecular functions are listed on the left. The size of the circle for each enriched molecular function is proportional to the significance. Only molecular functions with a corrected p-value < 0.05 are displayed (two-sided hypergeometric test, Bonferroni step down). A gene is considered significant if FDR < 0.001 and log2fold change > |0.2|.