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[Preprint]. 2023 Jan 22:2023.01.22.525052. [Version 1] doi: 10.1101/2023.01.22.525052

Figure 4. Nuclear pore component NPP-7 and the nucleolus promote piRNA dependent silencing.

Figure 4.

A) GFP expression in the piRNA reporter in indicated RNAi knockdowns. Asterisks indicate significant reporter activation, see Methods for details.

B) Small RNA fold changes in npp-7 RNAi knockdowns relative to empty vector control knockdown.

C) Fluorescent micrographs of live adult worm gonads show the localization of GFP::CSR-1, GFP::PRG-1, and GFP::PGL-1 in indicated RNAi knockdowns.

D) Fluorescent micrograph of individual live adult worm germline nucleus shows the localization of mCherry::NPP-7 and GFP::FIB-1. Top panels display a single slice through the nucleus center, bottom panels display average projection of all slices though the full nucleus volume. Percentage of soma and germline nuclei with nucleoli that contain NPP-7 are quantified to the right.

E) GFP expression in piRNA reporter in indicated RNAi knockdowns. Asterisks indicate significant reporter activation, see Methods for details.

F) Fluorescent micrographs of live adult worm gonads show the localization of GFP::CSR-1 in indicated RNAi knockdowns.