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[Preprint]. 2023 Jan 22:2023.01.22.525083. [Version 1] doi: 10.1101/2023.01.22.525083

Fig. 2 |. Enrichment of BAF increases nucleosome eviction.

Fig. 2 |

a, Comparison of promoter chromatin structure and chromatin accessibility by means of S5P CUTAC fragment size distribution over peaks (promoter and enhancer NDR spaces) in cells treated with DMSO (control) and Flavopiridol. Peaks were called with DMSO control. b, Heatmaps comparing nucleosomal (≥150 bp reads) and subnucleosomal (≤120 bp reads) protection by BAF (BRG1 CUT&RUN) and BAF-associated histones (BRG1 CUT&RUN.ChIP) in untreated (DMSO control) cells. Heatmaps were plotted relative to S5P CUTAC summits and sorted by decreasing accessibility (CUTAC signal). CUT&RUN.ChIP heatmaps show enrichment over IgG isotype control (for ChIP). c, d, Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤ 120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative to S5P CUTAC summits, in DMSO (c) and Flavopiridol treated (d) cells. e, Flavopiridol treatment causes eviction of partially unwrapped nucleosomes through enrichment of BAF at promoters and enhancers, leading to NDR persistence. Datasets are representative of at least two biological replicates.