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[Preprint]. 2023 Jan 18:2023.01.18.523029. [Version 1] doi: 10.1101/2023.01.18.523029

Figure 4. WSCR haploinsufficiency disrupts early communicative behavior.

Figure 4.

A) Cohort 3 was used to assess USVs at P5, 7, and 9, and the righting reflex at P14. B) Maternal separation induced pup USV workflow schematic: Remove dam, place pups in nest in warming chamber, individually measure pup temperature while in the nest, record USVs, then weigh and return to nest. C) Call rate was reduced in all groups compared to WTs overall (p=0.035). D-E) TG mice produced shorter duration calls across all three days, (D) with longer pauses between calls on P7 (E; TG > WT, TG/CD, p=0.020; CD > TG/CD, p=0.038). F) The mean pitch frequency of calls was lower on P5 and P7 for the TG/CD mice (p=0.012) and only P7 for the CD mice (p=0.011). G) The range of call frequencies was narrower at P7 for both the TG/CD and TG pups (p=0.038). H) At P5, the CD mice were unable to produce calls with similar sound pressure levels to WT pups (p=0.009). I) Pup weight after USV recordings show decreased weight in all pups with the CD allele (p=0.00007). J) Body temperature at recordings were comparable across groups. K) No differences were observed in latency to righting at P14 between groups. All statistical details including sample sizes are reported in Table S3.