FIG. 1.

Immunohistochemical staining with anti-total keratin and anti-involucrin antibodies. The stains were used to determine total keratin-positive and -negative cells, respectively, in HGEC-1 (A, B, G, and H), HeLa (C and D), and Gin-1 (E and F) cells. Cells were stained with mouse monoclonal antibody to human total keratin (AE1/AE3) (A, C, and E) and an isotype control, IgG1κ (B, D, and F). HGEC-1 were incubated with medium containing 2.0 mM (G) or 0.15 mM (H) calcium for 24 h or with mouse monoclonal antibody to human involucrin for involucrin detection.