TABLE 1:

Genes targeted for each virus and each assay

	SARS-CoV-2	Influenza A	Influenza B	RSV
SOC	E; RdRp*	M	M	M for RSVA/RSVB**
FAO	-	M	-	-
GX †	E; N2; RdRp	M; PA; PB2	M; NSP	N for RSVA/RSVB
TP ‡	N; S	M	M	N for RSVA M for RSVB
PC †	RdRp	M	NP	N for RSVA/RSVB

Legend: SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; IAV: Influenza A virus ; IBV: Influenza B virus ; RSVA/B: respiratory syncytial virus A/B; SoC: standard of care; FAO: Food and Agriculture Organization of United Nations; GX: Xpert^® Xpress SARS-CoV-2, Flu, RSV Kit; TP: TaqPath^™ COVID-19, FluA/B, RSV Combo Kit; PC: PowerChek^™ SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit II; E: Envelop; M: Matrix; N: nucleocapsid; NP: nucleoprotein; NSP: non-structural protein; PA: polymerase acidic protein; PB2: polymerase basic protein; RdRp: RNA-dependent RNA polymerase; S: spike.

MPAs target one, two or three genes for detection of each SARS-CoV-2, IAV, IBV and RSV.

^*SoC use two sets of primers and probes performed in two separate wells/PCR runs for each sample for detection of SARS-CoV-2

^**SoC use different optical channels to detect the RSV targets and then provide separate results for RSVA and RSVB

^†GX and PC use separate optical channels to detect SARS-CoV-2, IAV, IBV and RSV and provide results for each virus separately

^‡TP uses one optical detection channel for the detection of IAV and IBV and provides a combined result for influenza A/B, and similarly one optical channel is used for detection of RSVA and RSVB providing a combined result for RSV.