All metabolites in panel A came from random forest analysis between naïve and infected mice at 142 DPI. Bars are colored by superclass annotation generated in Classyfire, as implemented in MolNetEnhancer. (A) Metabolites impacted by infection. (B) Metabolites restored by any treatment (BNZ-only or combo treatment (BNZ+Tc24-C4/E6020-SE)). (C) Metabolites not restored by any treatment. (D) Metabolites only restored by BNZ + Tc24 vaccine treatment. (E) Metabolites only recovered by BNZ treatment. (F) Metabolites commonly restored by both treatments. RA, right atrium; LA, left atrium; LVT, left ventricle top; LVB, left ventricle bottom; RVT, right ventricle top; RVB, right ventricle bottom. N=15 mice per group and per position. (G) Representative metabolites restored by different treatments. red line, p-value <0.05 by Mann-Whitney U Test between two groups, FDR corrected.