Figure 3. Prostate-specific Dach1 gene deletion promotes prostate hyperplasia and dysplasia in OncoMice (15 weeks).

(A). Schematic representation of transgenes integrated into mice. (B). Representative immunohistochemistry for Dach1, with data quantitated as mean ± standard error of the mean (SEM) for N=20 (4 separate mice, with 5 views per mouse, in each group). (C). Blinded quantitative histology grading of prostate of multigenic mice at 15 weeks. Data are shown as mean ± SEM for N=15 (5 separate mice, with 3 prostate areas [anterior, ventral, lateral] per mouse) in each group). H&E staining demonstrates the presence of a focal atypical intraductal proliferation in Dach1^−/− prostate, compatible with prostatic intraepithelial neoplasia (PIN). Representative immunohistochemistry with results shown as mean ± SEM for Ki-67 (n=20, 4 separate mice for each genotype, 5 views per mouse) (D), Beclin 1 (n=9, 3 separate mice for each genotype, 3 views per mouse) (E); and AR (n=15 for Dach1^wt/wt mice, 3 separate mice, 5 views per mouse) (n=12 for Dach1^fl/fl mice, 3 separate mice, 2 views for one mouse and 5 views for other two mice) (F). Scale bars, 50 μm. A Student’s t-test was performed for all comparisons.