Skip to main content
[Preprint]. 2023 Jan 10:rs.3.rs-2423179. [Version 1] doi: 10.21203/rs.3.rs-2423179/v1

Figure 7. DACH1 enhances DNA repair.

Figure 7.

(A). LNCaP cells stably transduced with control vector or shDACH1 were treated with ATO (1μM), and immunofluorescence for 53BP1 or γH2AX was conducted. (B). The neutral pH comet assay, which mainly detects DNA double-strand breaks (DSBs), was conducted as a single-cell DNA damage assay. Dach1+/+ and Dach1−/− 3T3 cells were treated with 2 μM doxorubicin for 18 hrs. Scale bar, 100 μm with (C). data shown as mean ± SEM. (D). Schematic representation of DACH1 expression vectors, which were introduced into (E) U2OS cells expressing I-SceI based reporter assays for homologous repair (DR-GFP). Cells were analyzed after 48 hrs with data shown as mean ± SEM for N=12 (DACH1), N=7 (DACH1DDS), and N=5 (DACH1DC). (F). Dach1+/+ and Dach1−/− 3T3 cells or (G). Dach1−/− 3T3 transduced with a MSCV/DACH1-IRES-GFP expression vector or GFP vector control, were treated for 3 days with increasing doses of Talazoparib, a PARP inhibitor. Data are shown as mean ± SEM for N=3 separate experiments in triplicate.