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[Preprint]. 2023 Jan 20:rs.3.rs-2457013. [Version 1] doi: 10.21203/rs.3.rs-2457013/v1

Figure 7: Longevity of cellular immune responses induced by IN immunization.

Figure 7:

The durability of the antigen recall responses induced by immunization was measured by comparing the levels of secreted cytokines from splenocytes (SPL) or cervical lymph node isolates (cLN) from immunized mice in response to ex vivo stimulation with 5 μg RBD for 72h. SPL and cLN were isolated from young (8-wk old) mice given three IN immunizations with 10 μg RBD with either NE or NE/IVT either 2 or 25 wks after the last immunization (wk 10 or wk33, respectively). Levels of secreted cytokines were measured in the cell supernatant by multiplex immunoassay relative to unstimulated cells for (A) IFN-γ, (B) IL-2, (C) IP-10, (D) TNF-α, (E) IL-4, (F) IL-5, (G) IL-13, (H) IL-6, (I) IL-17A, and (J) IL-10. (n=5/grp; *p<0.05, **p<0.01 as compared between wk10 and wk33 for each adjuvant group by Mann-Whitney U test)