Figure 4. BMP9 increased the phosphorylation level of Smad1/5 and inhibited the phosphorylation level of ERK and JNK. WB showed that the overexpression of BMP9 inhibited the phosphorylation of ERK (A and D) and JNK (B and E) at 5 and 10 h and 2 and 5 h, respectively, and promoted the phosphorylation of Smad1/5 (C and F) in Pg-LPS-stimulated hDPCs. Immunocytofluorescence also showed that the overexpression of BMP9 inhibited the phosphorylation of ERK (G) and JNK (H) and increased the phosphorylation level of Smads1/5 (I). a: GFP+LPS; b: BMP9+LPS. Bars represent mean±SD (n=3). * p<0.05; ** p<0.01; *** p<0.001. scam bar: 75 μm.