Figure 5. Effects of BMP9 on the phosphorylation level of Smad1/5, ERK, and JNK within 1 h. At 5, 15 and 30 min after Pg-LPS stimulation, the phosphorylation level of ERK (A and D) and JNK (B and E) in the group treated with BMP9+LPS gradually decreased. The phosphorylation level of ERK (A and D) and JNK (B and E) in the group treated with GFP+LPS gradually increased, but it was significantly lower compared with the group treated with BMP9+LPS (WB). Under the same conditions, the phosphorylation level of Smad1/5 (C and F) in the group treated with BMP9+LPS did not significantly change at different time points and was significantly higher compared with the group treated with GFP+LPS. The phosphorylation level of Smad1/5 (C and F) in the group treated with GFP+LPS was weak at different time points (WB). Bars represent mean±SD (n=3). * p<0.05; ** p<0.01; *** p<0.001.