| Chemical compound, druC |
n-Dodecyl-β-D-Maltopyranoside |
Anatrace |
Cat#D310 |
|
| Chemical compound, drug |
Lauryl maltose neopentyl glycol |
Anatrace |
Cat#NG310 |
|
| Chemical compound, drug |
1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) |
Avanti Polar Lipids |
Cat#850457 C |
|
| Chemical compound, drug |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) |
Avanti Polar Lipids |
Cat#850457 C |
|
| Chemical compound, drug |
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) |
Avanti Polar Lipids |
Cat#840457 C |
|
| Chemical compound, drug |
Calcein |
Invitrogen |
Cat#C481 |
|
| Chemical compound, drug |
Fluo-4, Pentapotassium Salt, cell impermeant |
Invitrogen |
Cat#F14200
|
|
| Chemical compound, drug |
Valinomycin |
Sigma |
Cat#V0627 |
|
| Peptide, recombinant protein |
Hepcidin-25 (human) |
Sigma |
Cat#SML1118 |
|
| Commercial assay, kit |
TALON Metal Affinity Resin |
TaKaRa |
Cat#635504 |
|
| Commercial assay, kit |
SRT-10C SEC-300 |
Sepax Technologies |
Cat#239300–10030 |
|
| Commercial assay, kit |
Q Sepharose Fast Flow |
GE Healthcare |
Cat#17051010 |
|
| Commercial assay, kit |
Biobeads SM2 |
Bio-Rad |
Cat#1528920 |
|
| Commercial assay, kit |
PD-10 Desalting Column |
GE Healthcare |
Cat#17085101 |
|
| Commercial assay, kit |
400 nm NanoSizer Extruder |
T&T Scientific Corporation |
Cat#TT-004–0010 |
|
| Commercial assay, kit |
KOD Hot Start DNA polymerase |
Novagen |
Cat#71086–3 |
|
| Commercial assay, kit |
Fluo-4, AM, cell permeant |
Invitrogen |
Cat#F14201
|
|
| Commercial assay, kit |
pHrodo Red AM |
Invitrogen |
Cat#P35372
|
|
| Recombinant DNA reagent |
pGP-CMV-jGCaMP7s |
Addgene |
Plasmid #104463 |
RRID:Addgene_104463
|
| Software, algorithm |
MotionCorr2 |
Zheng et al., 2017
|
https://msg.ucsf.edu/em/software/motioncor2.html
|
|
| Software, algorithm |
Gctf |
Zhang, 2016
|
https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/
|
RRID:SCR_016500
|
| Software, algorithm |
Relion 3.0 |
Kimanius et al., 2016
|
https://www3.mrc-lmb.cam.ac.uk/relion
|
RRID:SCR_016274
|
| Software, algorithm |
CryoSPARC |
Punjani et al., 2017
|
https://cryosparc.com/
|
RRID:SCR_016501
|
| Software, algorithm |
ChimeraX |
Pettersen et al., 2021
|
https://www.cgl.ucsf.edu/chimerax/
|
RRID:SCR_015872 |
| Software, algorithm |
COOT |
Emsley et al., 2010
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
RRID:SCR_014222
|
| Software, algorithm |
PHENIX |
Adams et al., 2010
|
http://www.phenix-online.org/
|
RRID:SCR_014224
|
| Software, algorithm |
EMringer |
Barad et al., 2015
|
http://fraserlab.com/2015/02/18/EMringer/
|
|
| Other |
Grids: R1.2/1.3 Cu 300 mesh |
Quantifoil |
Cat#Q325CR1.3 |
Cryo-EM grid |
