Table 1. Summary of cryo-EM data collection, processing, and refinement.
| Sample | HsFpn-Ca2+–11 F9 |
|---|---|
| Cryo-EM data collection | |
| Voltage (kV) | 300 |
| Magnification (×) | 81,000 |
| Pixel Size (Å) | 1.10 |
| Electron exposure (e-/Å2/frame) | 1.25 |
| Defocus range (µm) | [-2.5,–0.8] |
| Number of image stacks | 4,498 |
| Number of frames per stack | 40 |
| Cryo-EM data processing | |
| Initial number of particles | 2,184,301 |
| Final number of particles | 437,959 |
| Symmetry imposed | C1 |
| Map resolution (Å) | 3.0 |
| Map resolution range (Å) | 2.4–3.6 |
| FSC threshold | 0.143 |
| Model refinement | |
| Number of amino acids | 875 |
| Total non-hydrogen atoms | 6,192 |
| Average B factor (Å2) | 171.4 |
| Bond length RMSD (Å) | 0.008 |
| Bond angle RMSD (°) | 0.939 |
| Ramachandran Plot | |
| Favored (%) | 93.53 |
| Allowed (%) | 6.24 |
| Outliers (%) | 0.23 |
| Rotamer outliers (%) | 1.25 |
| MolProbity Score | 1.98 |