Table 1.

Current approaches for detecting m7G in different types of RNAs

Technique	Throughput	Quantitative	RNA selection	Detection resolution	References
Northern blot	low	yes	tRNA	bulk level	Ma et al., 202141
Primer extension	low	no	rRNA	RNA fragment level	Zorbas et al., 201529
Immune-dot blot	low	no	mRNA, pri-miRNA	bulk level	Pandolfini et al., 201920
MeRIP-qPCR	low	yes	mRNA, pri-miRNA	RNA fragment level	Zhao et al., 202119
Liquid chromatography-electrospray ionization-tandem mass spectrometry	low	yes	mRNA	bulk level	Pandolfini et al., 201920
Enzyme treatment coupled with high-resolution mass spectrometry analysis	low	yes	mRNA	bulk level	Pandolfini et al., 201920
MeRIP-seq	high	yes	mRNA	RNA fragment level	Li et al., 202224
m7G-seq	high	yes	tRNA, mRNA	single-nucleotide level	Zhang et al., 202162
AlkAniline-seq	high	yes	tRNA, rRNA	single-nucleotide level	Marchand et al., 201833
TRAC-seq	high	yes	tRNA	single-nucleotide level	Lin et al., 201961
m7G-MaP-seq	high	yes	tRNA, rRNA	single-nucleotide level	Enroth et al., 201935