Table 2.
Selected epitopes for multi-epitope vaccine design.
| Protein | CTL epitopes (% rank, IC 50, antigenicity, immunogenicity) |
HTL epitopes (IFN-γ inducing activity, antigenicity) |
Linear B-cell epitopes (ABCpred score, antigenicity) |
|---|---|---|---|
| EspA | FIIDPTISA (0.089, 17092.26, 1.261, 0.107) |
DLTYIPVVGHALSAA (0.009, 0.554) |
YSEGAAAGTEDAERAP (0.970, 1.195) |
| EspA | TYIPVVGHAL (0.101, 390.63, 0.500, 0.195) |
ADGPVGAAAEQVGGQ (0.110, 1.395) |
EDAERAPVEADAGGGQ (0.920, 1.640) |
| EspA | / | TTTKKYSEGAAAGTE (0.010, 1.508) |
/ |
| Mpt63 | IPGYPVAGQVW (0.337, 416.95, 0.590, 0.033) |
STGKIYFDVTGPSPT (2.000, 0.823) |
GQVWEATATVNAIRGS (0.910, 0.466) |
| Mpt63 | GPSPTIVAM (0.030, 48.29, 1.149, 0.156) |
/ | / |
| LprA | KSEDAKFVY (0.070, 625.66, 1.288, 0.013), |
DAKFVYVDGHLYSDL (1.000, 0.403) |
GGCSTEGDAGKASDTA (0.930, 2.554) |
| LprA | NKSEDAKFVY (0.127, 980.34, 1.114, 0.027) |
/ | LVQIQIAPTKDTSVTL (0.930, 0.851) |
| PPE18 | VWGLTVGSW (0.353, 747.23, 0.689, 0.008) |
AAQVRVAAAAYETAY (0.335, 0.642) |
GARAGGGLSGVLRVPP (0.840, 1.358) |
| PPE18 | APAAAAQAV (0.087, 11.84, 0.638, 0.052) |
AMFGYAAATATATAT (0.604, 0.748) |
/ |
| PPE18 | TPAARALPL (0.015, 6.38,0.434, 0.123) |
QNGVRAMSSLGSSLG (0.458, 0.523) |
/ |
| EsxA | / | EQQWNFAGIEAAASA (2.000, 0.742) |
GGSGSEAYQGVQQKWD (0.860, 1.801) |
| EsxA | / | AGIEAAASAIQGNVT (2.000, 0.937) |
/ |
| EsxB | STNIRQAGVQY (0.224, 1137.27, 1.111, 0.107) |
AVVRFQEAANKQKQE (0.230, 0.858) |
LKTQIDQVESTAGSLQ (0.860, 1.039) |
| EsxB | / | STNIRQAGVQYSRAD (1.000, 0.999) |
/ |
| LppX | VLDPAAGVTQL (0.107, 4393.17, 0.426, 0.1090 |
QGVPFRVQGDNISVK (0.385, 1.992) |
SLLGITSADVDVRANP (0.950, 1.206) |
| LppX | HVAVRTTGK (0.217, 38.73, 0.994, 0.194) |
STTKITGTIPASSVK (1.000, 0.529) |
/ |
| LppX | RVQGDNISVK (0.155, 237.77, 2.643, 0.021) |
/ | / |
| EspC | SQFNDTLNV (0.185, 38.23, 0.616, 0.076) |
TAGVDLAKSLRIAAK (0.030, 0.670) |
AVDASSGVEAAAGLGE (0.890, 1.188 |
| EspC | SLHTAGVDLAK (0.385, 559.6, 1.024, 0.195) |
RIAAKIYSEADEAWR (0.027, 0.456) |
/ |