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. 2023 Jan 27;13:1554. doi: 10.1038/s41598-023-28768-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The phylogram of Blastocystis subtypes was inferred based on the nucleotide sequences of SSU rRNA gene. The evolutionary relationship of Blastocystis subtypes was constructed by the Maximum Likelihood method and Kimura 2-parameter model, based on the nucleotide sequences of SSU rRNA gene of Blastocystis isolated from stray cats [SC], household cats [C], and cat owners [H] in this study (blue circles) compared with nucleotide sequences of Blastocystis subtypes retrieved from GenBank, with Proteromonas lacertae (U37108) as outgroup. Bootstrap values obtained from 1000 replicates are indicated on branches in percentage; only bootstrap values > 50% are displayed. Evolutionary analyses were conducted in MEGA X.