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. 2022 Aug 17;30(1):111–124. doi: 10.1038/s41418-022-01049-1

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A HCT116 cells were irradiated at 4 Gy, and relative protein fold change of autophagy-related proteins LC3 and p62 were quantified. B Fluorescence images of HCT116 cells treated in (A) infected with LC3-GFP-RFP overexpressing lentivirus and subjected to 4 Gy radiation. Quantification of average dots per cell of RFP and GFP signals in each cellular condition was presented. Scale bars = 20 μm. n = 3. *P < 0.05, **P < 0.01 compared with the indicated group.