Figure 13.

Defective responses in myofibroblasts isolated from the colon of Nr1i2^-/-mice impart greater proteolytic activity of neutrophils. (A) Expression of genes related to neutrophil antimicrobial function. (B) IL1 family cytokines and (C) matrix metalloproteinases (MMPs) in the healing colon of mice with deletion of Nr1i2 in the mesenchymal compartment (Nr1i2^fl/fl vs Nr1i2^ΔCol1a2) or mice administered IPA after induction of colonic injury (n = 4 and n = 6 per group per respective experiment). (D) Pearson’s correlation of colonic expression of Il1b and MMP genes against the level of neutrophil infiltration into the colon of mice administered either H₂O or IPA water after induction of colonic injury. (E) Experimental schematic and (F) gene expression in bone marrow–derived neutrophils treated with supernatant from cytokine-stimulated colonic myofibroblasts isolated from WT or Nr1i2^-/ mice (n = 3 per group). Data are presented as mean ± standard error of the mean. Student t test (A–C, F). ∗P < .05, ∗∗P < .01, ∗∗∗P < .005; nd = no detection.