Figure 2.

Assessment of fibrotic remodeling in the colon after recovering from DSS-induced injury. (A) WT C57BL/6 mice were administered DSS (3%) for 5 days to induce colonic injury and inflammation and then switched to regular drinking water and allowed to heal for an additional 25 days. (B) Gross colon thickness was measured in mice without DSS and 10 and 25 days after withdrawal of DSS (after 5-day course). (C) Representative images of Masson’s trichrome staining (scale bar = 100 μm) and associated quantification of total fibrotic area and fibrotic thickness in the colon of control mice and mice administered DSS after healing for 25 days (n = 4 per group). Data are presented as mean ± standard error of the mean. Student t test (C). ∗∗∗P < .001.