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. 2022 Aug 22;30(1):137–151. doi: 10.1038/s41418-022-01051-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Representative fluorescence images of SLC7A11 and GPX4 proteins assessed by immunofluorescence assay in HCC clinical tissues from 12 radioresistant (HCC-R) and 12 radiosensitive (HCC-S) patients. Nuclei were stained with DAPI (×10, ×40). Scale bars, 50 μm. B Western blot assay of SLC7A11, GPX4 and tubulin proteins in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells at 4 h after 4 Gy IR or non-IR. Representative images (C) and quantification (D) of the relative fluorescence intensity of liperfluo (a lipid peroxide fluorescent probe) in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells at 4 h after 4 Gy IR or non-IR. Nuclei were stained with Hoechst (×40). Scale bars, 10 μm. E Dose responses of survival factions of SK-Hep-1 and HepG2 cells treated with RSL3, and the western blot assay of GPX4 and tubulin proteins in these cells. SK refers to SK-Hep-1, HG refers to HepG2. F, G Western blot assay of GPX4 and tubulin proteins in HCC cells treated with RSL3, siSOCS2 or its control siNC. H, I Dose responses of survival factions of SK-Hep-1 and HepG2 cells treated with RSL3, siSOCS2 or its control siNC. *P < 0.05, **P < 0.01 and ***P < 0.001 between indicated groups.