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. 2022 Aug 22;30(1):137–151. doi: 10.1038/s41418-022-01051-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Western blot assay of GPX4 and SOCS2 proteins and their relative levels in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells at 2, 4, 8, 24 h after 4 Gy IR or non-IR. B Western blot assay of SLC7A11, GPX4 and SOCS2 proteins in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells at 4 h after 4 Gy IR or non-IR. C, D Western blot assay of SLC7A11, GPX4 and SOCS2 proteins in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells with or without lvSOCS2 transfection at 4 h after 4 Gy IR or non-IR. Representative images (F) and quantification (E) of the relative fluorescence intensity of liperfluo in SK-Hep-1 and HepG2 cells transfected with lvSOCS2 at 4 h after 4 Gy IR or non-IR. Nuclei were stained with Hoechst (x40). Scale bars, 10 μm. *P < 0.05, **P < 0.01 and ***P < 0.001 between indicated groups.