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. 2022 Aug 22;30(1):137–151. doi: 10.1038/s41418-022-01051-7

Fig. 5. SOCS2 interacted with SLC7A11 and decreased its level by ubiquitin-proteasome pathway.

Fig. 5

A Representative immunofluorescence images of SOCS2 and SLC7A11 proteins in the nonirradiated xenograft tumors (SK-Hep-1 and SK-Hep-1R) and HCC clinical tissues. Nuclei are stained with DAPI (×10). Scale bars, 100 μm. B, C Co-immunoprecipitation and Western blot assay of SOCS2 and SLC7A11 proteins in the whole cell lysates of SK-Hep-1 and HepG2 cells at 4 h after 4 Gy IR. D, E Point-fold line chart of SLC7A11 protein degradation according to Western blot assay (Fig. S5E). F, G Western blot analysis of SLC7A11, SOCS2 and tubulin proteins in SK-Hep-1 and HepG2 cells at 4 h after 4 Gy IR. MG-132 (10 μM) or leupeptin (50 μM) were added before IR. H Anti-Ub immunoblotting assay of SLC7A11 polyubiquitination in SK-Hep-1 and Hep2 cells at 4 h after 4 Gy IR. I Anti-Ub immunoblotting assay of SLC7A11 polyubiquitination in SK-Hep-1 and HepG2 cells transfected with lvSOCS2 at 4 h after 4 Gy IR. *P < 0.05, **P < 0.01 and ***P < 0.001 between indicated groups.