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. 2022 Aug 16;30(1):125–136. doi: 10.1038/s41418-022-01050-8

Fig. 3. C106 of DJ-1 regulates the interaction with proteasome during ferroptosis.

Fig. 3

a–c Interaction between the endogenous DJ-1 with proteasome is abolished upon ferroptosis and is restored upon rescue. Whole-cell lysates were immunoprecipitated (IP) with the control IgG or anti-DJ-1 antibodies, and the precipitated proteins were detected by anti-PSMA4, anti-PSMB1 and anti-PSMD1 antibodies, respectively. d–f Interaction between the overexpressed DJ-1 and proteasome is disrupted upon ferroptosis and is restored upon rescue. g–i Mutation of C106A in DJ-1 abolishes its interaction with PSMB1 and PSMA4 but not with PSMD1. The whole-cell lysates in d–i were immunoprecipitated with anti-FLAG and precipitated proteins were detected by anti-PSMA4, anti-PSMB1 and anti-PSMD1. In a–i intensities of immunoblotting signals were quantified by ImageJ (NIH), and the quantitative ratios were calculated by dividing the intensity from the IP lane by that of the input lane. Relative band intensities are shown above each lane. Data was analyzed by student’s t-test. Error bars indicate standard deviations from triplicates. (**p < 0.01; ***p < 0.001; ****p < 0.0001; n.s. not significant, n = 3).