Fig. 6. MME⁺ FAPS are more prone to apoptosis after injury and become reduced after glycerol injury.

a, b Representative flow cytometric analysis (a) and quantification (b) of EdU⁺ MME^−/+ eGFP⁺ FAPs as a percentage MME^−/+ FAPs at 3 dpi in CTX injected (CTX^3dpi) or GLY (GLY^3dpi) injected muscle. c, d Representative flow cytometric analysis (c) and quantification (d) of the MME⁺ FAP fraction under baseline (BSL) conditions as well as 3 dpi in CTX (CTX^3dpi) injected or GLY (GLY^3dpi) injected muscle. e, f Representative flow cytometric analysis (e) and quantification (f) of c-caspase3⁺ FAPs as a percentage of MME^−/+ FAPs at 4 dpi in CTX injected (CTX^4dpi) or GLY (GLY^3dpi) injected muscle. g, h Representative flow cytometric analysis (g) and quantification (h) of the MME^−/+ FAP fraction under baseline (BSL) conditions as well as 28 dpi in CTX injected (CTX^28dpi) or GLY injected (GLY^28dpi) muscle. i Quantification of the number of mT⁺ cells remaining in mT⁺MME^- FAP-injected muscle (MME^-) or mT⁺MME⁺ FAP-injected muscle (MME⁺) at 28 dpi. j, k Representative flow cytometric analysis (j) and quantification (k) of the eGFP⁺ fraction of mT⁺ cells remaining in mT⁺MME^- FAP-injected muscle (MME^-) or mT⁺MME⁺ FAP-injected muscle (MME⁺) at 28 dpi. l, m Representative flow cytometric analysis (l) and quantification (m) of the MME⁺ FAP fraction in mT⁺MME^- FAP-injected muscle (MME^-) or mT⁺MME⁺ FAP-injected muscle (MME⁺) at 28 dpi. n, o Representative flow cytometric analysis (n) and quantification (o) of the MME⁺ FAP fraction at baseline (BSL) and in glycerol injected muscle at 70 dpi (GLY^70dpi). Each dot represents a single mouse. Bar graphs represent mean ± SEM. A two-way ANOVA with Tukey’s multiple comparisons test (ns p > 0.05, *p < 0.05, ****p < 0.0001) was used in b and f. A one-way ANOVA with Holm-Sidak’s multiple comparison test (*p < 0.05, ****p < 0.0001) was used in d and h. Student’s t test (two tailed, unpaired, *p < 0.05, **p < 0.01) was used in i, k, m, and o.